BIOLOGIYA MORYA, 2016, Vol. 42, No. 6, pp. 471-477

Ozonation and algalization as methods for control of microbial contamination of eggs of the Black Sea turbot Scophthalmus maeoticus Pallas, 1814 (Pisces: Scophthalmidae) during artificial incubation

© 2016 T. V. Rauen, V. S. Mukhanov, A. N. Khanaichenko

A.O. Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences, Sevastopol 299011

The methods of ozonation and algalization (addition of Chlorella vulgaris) were compared in terms of their efficiency in reducing bacterial contamination of Black Sea turbot egg incubation medium. The dynamics of abundance and the specific growth rate of bacteria in the egg incubation medium, as well as the intensity of egg colonization and the specific growth rate of bacterial r-strategists in M-65 medium (for the evaluation of their contribution to the bacterial community) were assessed in treatments and control. Ozonation had a short-term inhibitory effect on the incubation medium microflora, followed by an intense selective growth of opportunistic bacteria. On the contrary, algalization formed a microbially balanced and safe incubation medium for the Black Sea turbot eggs and reduced their bacterial colonization.

Key words: Scophthalmus maeoticus, turbot eggs, artificial incubation, incubation medium, bacterial contamination, ozonation, algalization, Chlorella.