BIOLOGIYA MORYA, 2016, Vol. 42, No. 3, pp. 224-230 |
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Seasonal dynamics of diarrhetic shellfish poisoning toxins in digestive gland of Crenomytilus grayanus (Dunker, 1853) analyzed by high-performance liquid chromatography with fluorometric detection 1A.V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041; E-mail: The seasonal dynamics of diarrhetic shellfish poisoning (DSP) toxins in the digestive gland of the mussel Crenomytilus grayanus (Dunker, 1853) from Peter the Great Bay, Sea of Japan, have been studied. Toxins in form of 4-bromomethyl-7-methoxycoumarin derivatives have been analyzed using high-performance liquid chromatography (HPLC) with fluorometric detection. This method allows determination of the quantitative and qualitative composition of individual toxins. All the C. grayanus samples contained DSP toxins, but their concentration varied substantially during the year. The highest concentration was recorded from the mussels collected in May; the lowest, in February. The toxins were represented predominantly by dinophysistoxin-2; in July-September and December, okadaic acid was detected. An assumption has been made about the relationship between the potential producers of DSP toxins in the waters of Peter the Great Bay and the dynamics of these toxins in tissues of C. grayanus. The HPLC method, as an alternative to enzyme-linked immunosorbent assay, has been applied for assessing the quantitative and qualitative composition of DSP toxins in seafood for the first time in the Russian Federation. This method can be recommended for the routine monitoring of toxins conducted by the controlling organizations of the Russian Federal Service for Veterinary and Phytosanitary Surveillance. Key words: diarrhetic shellfish poisoning toxins, okadaic acid, dinophysistoxin-2, Crenomytilus grayanus, HPLC, fluorometric detection. |
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