BIOLOGIYA MORYA, 2016, Vol. 42, No. 2, pp. 99-103 |
|
Black Sea algal viruses Institute of Natural and Technical Systems, Russian Academy of Sciences, Sevastopol 299011 E-mail: Monitoring of the Black Sea algal viruses in bays near Sevastopol and at the coast of Crimea has been carried out since 2002. Using the methods developed and patented by the author, more than 200 strains of new for science algal viruses of five species of microalgae were isolated: TvV (Tetraselmis viridis), DvV (Dunaliella viridis virus), PtV (Phaeodactylum tricornutum virus), PpV (Prorocentrum pusillum virus) and IgV (Isochrysis galbana virus). In the Black Sea, a virus of the microalga Emiliania huxleyi (EhV) was isolated for the first time. Using the method of electron microscopy, the Black Sea algal viruses were identified as icosahedral virions with a respective size of 56-60, 45-48, 50-53, 88-92 and 128-132 nm, for the TvV, PtV, DvV, PpV and IgV. The EhV size determined by the method of filtration was within the range of 50-200 nm. In the IgV and EhV viruses, we revealed the presence of viral envelope. Based on their characters, the isolated algal viruses were referred to the Phycodnaeviridae. The maximum number of algal viruses was observed in the spring and autumn, which is typical for their host phytoplankton species. The Black Sea algal viruses, TvV, PpV, IgV and EhV, showed no strict species-specificity and have a wide range of hosts. Key words: algal viruses, microalgae, Black Sea. |
|
|