BIOLOGIYA MORYA, 2015, Vol. 41, No. 5, pp. 324-330 |
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Dynamics of intracellular content of carbon, nitrogen and chlorophyll a under conditions of batch growth of the diatom Phaeodactylum tricornutum (Bohlin, 1897) at different light intensities A.O. Kovalevsky Institute of Marine Biological Research, Russian Academy of Sciences, Sevastopol 299011 E-mail: During batch growth of the diatom Phaeodactylum tricornutum (Bohlin, 1897), the specific content of nitrogen in the cells (carbon-based estimates) decreased with lowering nitrogen concentration in the medium below ∼10 µM/L. When nitrogen of the medium was exhausted, the microalga continued to grow for some time by using the intracellular reserve of this element. The C/N ratio in the cells increased from 5 to 14, and the density of culture (carbon-based estimates) increased about two-fold. The relationship between the specific growth rate of P. tricornutum and the C/N ratio in the cells was described by an equation similar to the Droop (1968) model. The ratio of intracellular concentrations of carbon to chlorophyll a (C/Chl a) increased exponentially with a decrease in the specific nitrogen content in the cells; this effect was enhanced with increasing light intensity. The value of the minimum cell quota of nitrogen was not dependent on the light conditions of algal growth. Key words: marine diatoms, Phaeodactylum tricornutum, specific growth rate, C/Chl a ratio, C/N ratio, light intensity. |
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