V. V. Besprozvannykh, D. M. Atopkin, H. D. Ngo, A. Yu. Beloded, A. V. Ermolenko, N. V. Ha, N. V. Tang - The trematode Skrjabinolecithum spasskii Belous, 1954 (Digenea: Haploporidae), a parasite of mullets (Mugilidae) from Peter the Great Bay of the Sea of Japan and from Vietnam waters of the Gulf of Tonkin: morphology and molecular data

BIOLOGIYA MORYA, 2015, Vol. 41, No. 4, pp. 256-263

The trematode Skrjabinolecithum spasskii Belous, 1954 (Digenea: Haploporidae), a parasite of mullets (Mugilidae) from Peter the Great Bay of the Sea of Japan and from Vietnam waters of the Gulf of Tonkin: morphology and molecular data

¹Institute of Biology and Soil Science, Far East Branch, Russian Academy of Sciences, Vladivostok 690000;
²Department of Cell Biology and Genetics, Far Eastern Federal University, Vladivostok 690091;
³Institute of Ecology and Biological Resources, Vietnamese Academy of Sciences and Technology, Hanoi, Vietnam 10000

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The trematode Skrjabinolecithum spasskii was described by Belous, from the redlip mullet Mugil soiuy (=Liza haematocheila) from the Razdolnaya (Suifun) River (southern Far East of Russia). In the description of the species the author noted that adult S. spasskii had no pigment eye-spots, but had a spherical testis and short caeca; the uterus contained eggs with miracidia devoid of an eye-spot. We found adult worms of this species in the intestine of the redlip mullet Liza haematocheila and the striped mullet Mugil cephalus from Peter the Great Bay of the Sea of Japan and in the striped mullet M. cephalus from Vietnam waters of the Gulf of Tonkin. Our study of the morphology of S. spasskii showed that the worms had diffuse eyes-spot, V-shaped testis, long caeca reaching the level of the middle of the last third of the body, and the uterus with unembryonated underdeveloped eggs with an operculum on one pole and a knob on the other pole. The miracidium develops on eggs hatched in water and has an eye-spot. An analysis of the nucleotide sequences of the 28S rRNA gene and the fragment ITS1-5.8S-ITS2 revealed intraspecific differentiation of the three genotypes of S. spasskii, two of them were detected in the trematodes from Primorye Region and one genotype in the trematodes from Vietnam.

Key words: Skrjabinolecithum spasskii, DNA, 28S rRNA gene, Haploporidae, Mugilidae, trematodes.