BIOLOGIYA MORYA, 2015, Vol. 41, No. 2, pp. 122-129

Fluorescent in vitro phagocytosis assay differentiates hemocyte activity of the bivalve molluscs Modiolus kurilensis (Bernard, 1983) inhabiting impacted and non-impacted water areas

© 2015 Yu. N. Sokolnikova¹, E. V. Trubetskaya¹, I. A. Beleneva², A. V. Grinchenko¹, V. V. Kumeiko^{1, 2}

¹Far Eastern Federal University, Vladivostok 690950;
² A.V. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok 690041

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In vitro hemocyte phagocytosis assay revealed no significant differences in the average values of phagocytic activity (PA) and phagocytic index (PI) in samples of the molluscs Modiolus kurilensis inhabiting non-impacted water areas (Kievka, Vostok, and Troitsa bays, the Sea of Japan). The only exception was an insignificantly lower value of PI for individuals from Troitsa Bay. The average values of PA for hemocytes of the total population of bivalves from non-impacted water areas were 52.0 ± 1.0%. On the basis of the interannual analysis of PA, the bivalve population from Vostok Bay was chosen as an appropriate reference group characterized by relatively stable PA values. In comparison with molluscs from reference group and other investigated samples, M. kurilensis from the impacted water area (Sportivnaya Gavan, Amursky Bay) had considerably lower values of average PA (36.0 ± 2.0%) and PI (2.1 ± 0.05). The presented cell-mediated immunity assay can serve as an effective method for estimation of bivalve physiological state in natural and aquacultural populations in order to differentiate molluscs from impacted and non-impacted water areas.

Key words: innate immunity, phagocytosis, bivalve molluscs, polluted and non-polluted water, hemolymph.