N. S. Vorobyeva, A. N. Mazeika, L. A. Davydova, P. V. Velansky, А. V. Tsybulsky, E. Y. Kostetsky, N. M. Sanina - Effect of triterpene glycosides and phospholipids from marine invertebrates in the composition of tubular immunostimulating complexes on immunogenicity of human serum albumin

BIOLOGIYA MORYA, 2015, Vol. 41, No. 1, pp. 64-71

Effect of triterpene glycosides and phospholipids from marine invertebrates in the composition of tubular immunostimulating complexes on immunogenicity of human serum albumin

Far Eastern Federal University, Vladivostok 690091

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To study adjuvant activity and to optimize tubular immunostimulating complexes (TI-complexes) with respect to a water-soluble antigen, the investigation was performed on cucumariosides and their fractions, i.e. individual triterpene glycosides obtained from the holothurian Cucumaria japonica, as well as on phospholipids from the sea urchin Strongylocentrotus intermedius and the starfish Distolasterias nippon in the composition of TI-complexes in order to assess their effect on the immunogenicity of human serum albumin (HSA). As a whole, TI-complexes showed a moderate adjuvant activity with respect to HAS; this activity can be adjusted by substitution of a cucumarioside and a polar lipid in the composition of the TI-complex. So, TI-complexes based on monogalactosyldyacylglycerol (MGDG) from the sea alga Ulva lactuca and cucumariosides A₂-2 and A₄-4 maximally stimulated anti-HSA antibody production in mice. Substitution of MGDG for phosphatidylcholine (PC) from the starfish D. nippon with an increased (compared with other investigated phospholipids) ratio of n-3/n-6 polyunsaturated fatty acids did not result in a change of immunostimulating activity of the TI-complex. However, the use of other phospholipids led to a decrease in the adjuvant activity of the TI-complex, compared with that of the TI-complex based on PC from D. nippon and cucumarioside A₂-2. The largest fluctuations in the contents of cytokines IL-6, IL-12, IFN-γ and GM-CSF depending on the composition of TI-complexes indicate the possibility of regulation of the T-cell immune response. The values of hematological parameters were normal.

Key words: immunostimulating complexes, cucumariosides, phosphatidylcholine, phosphatidylethanolamine, Cucumaria japonica, Strongylocentrotus intermedius, Distolasterias nippon, antibodies, cytokines, hematology.