BIOLOGIYA MORYA, 2014, Vol. 40, No. 5, pp. 384-390

The development of Porphyridium purpureum (Bory de Saint-Vincent, 1797) Drew et Ross, 1965 (Rhodophyta) from Amursky Bay, Sea of Japan, in a laboratory culture

© 2014 N. A. Aizdaicher¹, I. V. Stonik¹, A. V. Boroda^{1, 2}

¹A.V. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok 690041;
²Far Eastern Federal University, Vladivostok 690950

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This paper examines the peculiarities of growth in culture of the red microalga Porphyridium purpureum on different media and at different water salinity. The ability of this species to recover after cryopreservation was studied. The growth rates and generation time of P. purpureum at the exponential stage were not substantially different between algae cultured on 'f' medium and Goldberg's medium. The growth dynamics was stable at salinity ranging from 8 to 32‰. Cell cryopreservation experiments using a three-step freezing procedure and trehalose as the cryoprotectant showed that the cell growth dynamics of P. purpureum after thawing differed insignificantly from those of the control.

Key words: Porphyridium purpureum, laboratory culture, salinity, cryopreservation.