BIOLOGIYA MORYA, 2014, Vol. 40, No. 5, pp. 373-383

A genetic and ultrastructural study of three clones of Porphyridium purpureum (Bory de Saint-Vincent, 1797) Drew et Ross, 1965 (Rhodophyta) from the marine microalgae collection of the A.V. Zhirmunsky Institute of Marine Biology

© 2014 K. V. Efimova, M. A. Kreshchenovskaya, N. A. Aizdaicher, T. Yu. Orlova

A.V. Zhirmunsky Institute of Marine Biology, Far East Branch, Russian Academy of Sciences, Vladivostok 690041

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In this paper, we present the results of a comparative genetic and ultrastructural study of three clones of the microalga Porphyridium purpureum (Rhodophyta) from the collection of marine microalgae cultures of the A.V. Zhirmunsky Institute of Marine Biology. All clones, which have different geographical origin, showed a high similarity in terms of both the ultramicroscopic structure and the nucleotide sequences of the nuclear ribosomal DNA genes (18S rDNA, ITS1-5.8S rDNA-ITS2, D1-D2 region of 28S rDNA). The obtained data are very helpful for the certification of two strains of P. purpureum, which were isolated for the first time in the practice of Russian algological research.

Key words: laboratory culture, microalgae, Porphyridium purpureum, ribosomal genes, ultrastructure.