BIOLOGIYA MORYA, 2014, Vol. 40, No. 1, pp. 63-69

Invertebrates of the Sea of Okhotsk as a new source of lypopolysaccharide-binding proteins

© 2014 S. I. Bakholdina¹, G. A. Naberezhnykh¹, V. I. Gorbach¹, M. P. Isaeva^{1, 2}, T. F. Solov'eva¹

¹Elyakov Pacific Institute of Bioorganic Chemistry, Far East Branch, Russian Academy of Sciences, Vladivostok 690022;
²Far Eastern Federal University, School of Biomedicine, Vladivostok 690950

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Invertebrates from the Sea of Okhotsk were studied as a source of proteins that are capable of binding to lipopolysaccharides (LPS) of gram-negative bacteria. DOT-analysis using dansyl-labeled LPS developed by us revealed LPS-binding proteins in blood cell lysates of 21 out of 33 investigated species of invertebrates. Most of the investigated species with positive LPS-binding activity were decapod crustaceans (class Malacostraca, phylum Arthropoda). Hemocyte lysates from the crab Paralithodes camtschaticus and the shrimp Sclerocrangon boreas contained several LPS-binding proteins with different molecular mass. LPS-binding proteins were found in echinoderms (classes Holothuroidea, Asteroidea, and Echinoidea), sipunculans, and brachiopods for the first time.

Key words: marine invertebrates, innate immunity, LPS-binding proteins, Sea of Okhotsk.